4.Journals Published in Sri Lanka
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Item The effects of the basic medium and the carbohydrate content on shoot cultures of Hevea brasiliensis(Rubber Research Institute. Agalawatte, 1996) Seneviratne, P.; Flegmann, A.W.; Wijesekera, G.A.S.Woody Plants Medium (WPM) was superior to murashige and skoog medium (M S), which showed better axillary bud growth of both juvenile and mature shoot materials. The effect was more pronounced in later passages, in particular, on the survival rate of the cultures. Most of the cultures grown on M S medium did not survive more than 24-28 weeks, while on WPM medium cultures survived for more than one and a half years, until the experiment was terminated. The level of sucrose in the medium also had an effect on the growth of the primary axillary shoots, specially the length and the leaf growth. The length of primary axillary shoots incresed with the increasing levels of sucrose in the medium up to 10 per cent with clonal materials. Explants did not survive beyond 8 weeks when the medium was not supplied with sucrose.Item The positional effect of the explant on In vitro growth of axillary buds of Hevea brasiliensis(Rubber Research Institute. Agalawatte, 1996) Senaviratne, P.; Flegmann, A.W.; Wijesekera, G.A.S.Apart from the high apical dominance showed in shoot explants of hevea, the location of the node in the shoot, seems to play a vital role in in vitro axillary bud proliferation. Better performance was observed when the axillary buds harvested as nodes, were not too close and also not too far from the apex as measured by the primary axillary shoot growth. This was true for both juvenile and mature arigin materials. Furthermore, "active" nodes were superior to "dormant" nodes.