Tissue culture of Innala (Solenostemon rotundifolius (POIR.) J.K.MORTON) High frequency plant regeneration from leaf examples

Abstract

Leaf explants of innala (Solenostemon rotundifolius (Poir.)J.K.Morton) were cultured on media prepared with MS salts,organic constituenets of NN,sucrose (40g/IW/V)and growth regulators.NAA,2,4-D,BAP andKn were used as growth regulators. Callus formation and subsequent shoot regeneration from cultured explants were observed in the presence of 0.8-1.0 mg/I BAP in the medium. When low concentration (0.5mg/I) of NAA was present in the medium, a higher level(5-6mg/I) of BAP was required for induction of shoots. The frequency of callus formation or shoot regeneration in such was as high as 100.The regenerated shoots could be rooted in hormone-free MS medium and successfully established in soil.