4.Journals Published in Sri Lanka
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Item In vitro shoot proliferation of rosewood (dalbergia latifolia: fabaceae): a potent high quality timber species(Faculty of Agriculture, University of Ruhuna:Kamburupitiya, 2015) Subasinghe, S.; Warakagoda, P.S.; Abeywickrama, W.H.T.Item In vitro propagation of Hemidesmus indicus (L) R.Br. (Iramusu) through nodal culture(Postgraduate Institute of Agriculture, University of Peradeniya:Peradeniya, 2007) Nagahatenna, D.S.K.; Peiris, S.E.Item Amylase and Glucosidase enzyme inhibitory activity of ginger (Zingiber officinale Roscoe ) an in vitro study(Postgraduate Institute of Agriculture, University of Peradeniya:Peradeniya, 2007) Abeysekera, W.K.S.M.; Chandrasekera, A.; Liyanage, P.K.Item In vitro flower induction in Gerbera (Gerbera jamesonii Adlam)(Postgraduate Institute of Agriculture, University of Peradeniya:Peradeniya, 2006) Ranasinghe, R.A.T.D.; Abayagunawardana, A.G.N.I.; Hettiarachchi, H.I.D.D.; Farzana, A.R.F.; Eeswara, J.P.Item The effects of the basic medium and the carbohydrate content on shoot cultures of Hevea brasiliensis(Rubber Research Institute. Agalawatte, 1996) Seneviratne, P.; Flegmann, A.W.; Wijesekera, G.A.S.Woody Plants Medium (WPM) was superior to murashige and skoog medium (M S), which showed better axillary bud growth of both juvenile and mature shoot materials. The effect was more pronounced in later passages, in particular, on the survival rate of the cultures. Most of the cultures grown on M S medium did not survive more than 24-28 weeks, while on WPM medium cultures survived for more than one and a half years, until the experiment was terminated. The level of sucrose in the medium also had an effect on the growth of the primary axillary shoots, specially the length and the leaf growth. The length of primary axillary shoots incresed with the increasing levels of sucrose in the medium up to 10 per cent with clonal materials. Explants did not survive beyond 8 weeks when the medium was not supplied with sucrose.Item The positional effect of the explant on In vitro growth of axillary buds of Hevea brasiliensis(Rubber Research Institute. Agalawatte, 1996) Senaviratne, P.; Flegmann, A.W.; Wijesekera, G.A.S.Apart from the high apical dominance showed in shoot explants of hevea, the location of the node in the shoot, seems to play a vital role in in vitro axillary bud proliferation. Better performance was observed when the axillary buds harvested as nodes, were not too close and also not too far from the apex as measured by the primary axillary shoot growth. This was true for both juvenile and mature arigin materials. Furthermore, "active" nodes were superior to "dormant" nodes.Item Somatic embryogenesis for hevea. the technique of anther culture(Rubber Research Institute. Agalawatte, 1996)Production of pollen plants via somatic embryogenesis might be cultivar dependant as successful production of pollen plants is reported only by chinese scientists. None of the locally available clones of hevea produced even somatic embryos. The slow progress made on adoptation of this technique suggests difficulties in reproducing, may be due to various critical factors involved in the process. The rate of callus formation was influenced by the amount of auxins an d cytokinins in the medium and the presence of both types was important for initiation of callus.Item In vitro interactions between Rigidoporus lignosus, the case of white root disease of rubber and some potentially antagonistic fungi(Rubber Research Institute of Sri Lanka. Agalawatte, 1995) Jayasuriya, K.E.; Deacon, J.W.The modes of aaction of fungi potentially antagonistic against R. lignosus were assessed in vitro by studying their ability (a)to cause pre- or post-contact damage to R. lignosus hyphae in video taped interactions on thin purified water agar films and, (b) to displace the established pathogen from woody inocula. The most fungi were Trichoderma spp. which produced volatile or non-volatile inhibitors. Basidiomycetes such as Trametes sp. caused hyphal interface similar to that of Phlebiopsis gigantea, a commercial biocontrol agent of Heterobasidion annosum in pine stumps. species of Trametes and Aspergillus partially or completely displaced R. lignosus from previousley colonized woody inocula.Item In vitro antagonism caused by some species of fungi on Rigidoporus lignosus(Rubber Research Institute. Agalawatte, 1996) Jayasuriya, K.E.; Deacon, J.W.; Fernando, T.H.P.S.Fungi potentially antagonistic to rigidoporus lignosus were isolated from a) by placing soil samples on agar plates previously colonized by R.lignosus, or on trichoderma selective agar and b) from basidiomycete fruit bodies in rubber plantations.Modes of action of potential antagonists against R. lignosus were evaluated by in vitro methods viz: production of volatile or non-volatile inhibitory compounds and ability to overgrow colonies of R. lignosus an agar. Some trichoderma spp. were strongly antagonistic to R. lignosus in most of the tests. T. longibrachiatum (isolate DZE10) completely inhibited R. lignosus an agar by producing volatile inhibitory metabolites. Trametes sp. producing volatile inhibitory metabolites. Trametes sp. (isolates ISO16 KIR8) antagonized R. lignosus on dual membered plates.Item The effect of thidiazuron on axillary shoot proliferation of Hevea brasiliensis in vitro(Rubber Research Institute of Sri Lanka. Agalawatte, 1996) Senaviratne, P.; Flegmann, A.W.Multiple axillary shoot production with a shoot doubling time of 26 days was obtained from nodal explants of juvenile orgin Hevea brasiliensis on a medium containing thidiazuron (N-phenyl- N'1,2,3-thidiazol-5ylyrea) at 0.02 ppm in combination with NAA at 0.2 ppm. The clusters of axillary buds produced in the presence of thidiazuron were subcultured at 4-week intervals while subdividing into two to four small propagules depending on the size of the cluster. Axillary buds showed a satisfactory elongation on their transfer onto a growth regulator-free medium. Elongated axillary shoots which were more than 100 mm in hight produced roots in the presence of IBA at 2 ppm.