Production of cell wall degrading enzymes by Corynespora cassiicola in culture and infected rubber tissue

Abstract

All isolates of Corynespora cassiicola used in the investigation secreted pectin lyase when grown in liquid medium with pctin as the main source of carbon. However, none of the isolates secreted polygalacturonase in the same medium. The extracts of rubber tissue inoculated and infected with C. cassiicola also showed only pectin lyase activity. When grown in liquid medium with carboxymethyl cellulose as the main source of carbon all isolates secreted cellulases viz. cellobiase and ß - glucosidase. The result of this study suggest that principal pectic enzyme involved in pathogenesis of the rubber isolates of C. cassiicola is pectin lyase and in the later stages cellulolytic enzymes possibly play a major role