A rapid minipreparation method for DNA extraction from Hevea leaf tissue

Abstract

Use of DNA markers in plant breeding is increasingly becoming popular. A basic requirement in using this technique in plant breeding is the availability of a simple inexpensive method for the isolation of plant DNA from large populations. Here we describe a rapid method of DNA isolation from Hevea leaf tissue. DNA yields obtained by this method were comparable with the yields of the other available methods. The quality of the DNA was tested by digesting with several restriction enzymes and was found to be of adequate purity. Southern blot analysis of this DNA showed its suitability for RFLP analysis of Hevea.