A rapid minipreparation method for DNA extraction from Hevea leaf tissue

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dc.contributor.authorHerath, S.en_US
dc.contributor.authorAttanayaka, D.P.S.T.G.en_US
dc.contributor.authorKarunanayake, E.H.en_US
dc.date.accessioned2012-06-25T08:21:50Z
dc.date.available2012-06-25T08:21:50Z
dc.date.issued1996en_US
dc.description.abstractUse of DNA markers in plant breeding is increasingly becoming popular. A basic requirement in using this technique in plant breeding is the availability of a simple inexpensive method for the isolation of plant DNA from large populations. Here we describe a rapid method of DNA isolation from Hevea leaf tissue. DNA yields obtained by this method were comparable with the yields of the other available methods. The quality of the DNA was tested by digesting with several restriction enzymes and was found to be of adequate purity. Southern blot analysis of this DNA showed its suitability for RFLP analysis of Hevea.en_US
dc.identifier.citationJournal of the Rubber Research Institute of Sri Lanka78pp.44-51en_US
dc.identifier.urihttps://dl.nsf.gov.lk/handle/1/8961
dc.publisherRubber Research Institute. Agalawatteen_US
dc.subjectBotanyen_US
dc.subjectDNAen_US
dc.subjectHeveaen_US
dc.subjectLeaf tissuesen_US
dc.titleA rapid minipreparation method for DNA extraction from Hevea leaf tissueen_US
dc.typeArticleen_US

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